All India Institute Of Medical Sciences

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  1. Ramyavani Errol says:

    Ramyavani Errol
    King Faisal Specialist Hospital & Research Centre
    Department of Pathology and Laboratory Medicine
    Molecular Genetics
    MBC #10, PO 3354
    Riyadh-11211
    Saudi Arabia
    Phone: +9661-4647272 ext 35890
    Mobile: (00966) 507949239, 559880241
    Res #: +9661-2120196
    agnesramya@yahoo.co.in

    PROFILE Research Assistant and Medical laboratory technologist in the field of research and diagnostic molecular biology techniques, having more than 7 yrs of experience.
    WORK HISTORY
    June 2008– Till date Medical Laboratory Technologist
    King Faisal Specialist Hospital & Research Center
    Department of Pathology and Laboratory Medicine Molecular Genetics Laboratory
    Riyadh, Saudi Arabia.

    Responsibilities

     Genetic Screening of Genetic Disorders in Humans
    Like Ret,MTHFR,HFE,SICLE CELL, CYSTIC FIBROSIS, B-THAL, AND MANY MORE ALSO INCLUDES CANCER GENETIS.
     Extraction and Purification of DNA/RNA from the blood , tissue,cord blood, Amniotic fluid, Chorionic Villus, using automated extractors eg. MagNapure (Roche), and manual extraction using DNAZol.
     PCR for Amplification of the DNA.
     Gel Electophoresis.
     Sequence Reaction, and Purifying the products
     Sequencing the Amplified product using ABI 3730 and ABI 3100 DNA Analyser.
     Gene Mapping, Mutation analysis etc.
     General
     Perform Quality Control testing for all diagnostic tests done in the laboratory.
     Evaluation of new diagnostic equipments when ever required.
     Standardization of molecular diagnostic methodologies to obtain optimal results.
     Media and reagent preparation.
     Maintaining daily check of stock and supplies for the Laboratory.
     Calibration of equipments according to schedule and as and when required.
     Daily maintenance of the laboratory according to CAP recommendation.

    • Working knowledge of the following equipments/instruments.
     MagnaPure for Nucleic acid extraction
     Roche Light Cycler
     MJ Research ,GeneAmp, BioRad, Gradient ,Thermal Cyclers
     ABI 3730 and ABI 3100 DNA Analysers for Sequence Analysis and Gene Mapping.
     CERNER Operation
     Gel Documentation System

    October 2004 – July 2007 Medical Laboratory Technologist
    King Faisal Specialist Hospital & Research Center
    Department of Pathology and Laboratory Medicine Diagnostic Molecular Pathology
    Riyadh, Saudi Arabia

    Responsibilities

    • Molecular diagnostics
     Extraction and Purification of DNA/RNA from Viruses such as HIV, CMV, HBV, HCV, ENT, HSV, EBV etc, using automated extractors eg. MagNapure (Roche) and M2000 sp (Abbott).
     Multiplex PCR for identification of Atypical pneumonia(Clymadia pneumonia, Mycoplasma pneumonia, pneumocystic caranii, Legionella pneumophilla .
     Detection of respiratory viruses using Multiplex PCR.
     Detection of Human Herpes viruses and Adino viruses using light cycler.
     Identification of viral DNA using qualitative and quantitative PCR (Real time PCR (Roche) and MJ Research master cyclers.).
     Quantification of viruses using Branch DNA (Bayer), ABBOTT and ABI 7000.
     Typing (genotyping and drug resistant) for HIV, Hep B and Hep C.( INNOLIPA).
     Mycobacterium species identification using DNA probe technique.
     Molecular diagnosis of Chlamydia and Mycoplasma pneumoniae
     RT-PCR for the diagnosis of avian influenza (H5N1) virus.
     MRSA agglutination test.
     Agarose gel electrophoresis.
     Gel documentation system.
     Reporting results using CERNER.
     Analyzing CAP samples for External Proficiency Testing.

    • General
     Perform Quality Control testing for all diagnostic tests done in the laboratory.
     Evaluation of new diagnostic equipments when ever required.
     Standardization of molecular diagnostic methodologies to obtain optimal results.
     Media and reagent preparation.
     Maintaining daily check of stock and supplies for the Laboratory.
     Calibration of equipments according to schedule and as and when required.
     Daily maintenance of the laboratory according to CAP recommendation.

    • Working knowledge of the following equipments/instruments.
     MagnaPure for Nucleic acid extraction
     Roche Light Cycler
     MJ Research Thermal Cycler
     BAYER branch DNA for Viral Qantification
     ABBOTT and ABI 7000 for Viral Quantification
     InnoLiPa for Genotyping and Drug Resistance Typing for Viruses
     CERNER Operation
     Gel Documentation System

    October 2000 – October 2004 Research Assistant
    Vector Control Research Centre
    Department of Molecular Biology & Genetics
    Pondicherry-605006
    India.

    Responsibilities

    • Extraction, purification and Identification of DNA/RNA of diseased vector/parasites and also from human blood.
    • PCR(Eppendorf and MJ Research) Techniques for Amplification of target DNA/RNA
    • RAPD (Random amplified polymorphic DNA) analysis AFLP (Amplified fragment length polymorphism) analysis.
    • RT-PCR (reverse transcriptase-PCR) for the studies of Dengue virus and to obtain c-DNA.
    • Using ELISA techniques.
    • Electrophoresis: -Agarose gel Electrophoresis for DNA,
    -SDS- PAGE for Protein -PAGE for manual sequencing
    of DNA and other Radio label experiments.
    • Techniques involved in c DNA cloning.
    • Preparation of media solution involved in both molecular and microbiological studies.
    • Using computer software for population genetic analysis.
    • Working knowledge of HPLC, Gel documentation system and Radio label studies.
    PUBLICATON
    AND PROJECTS
    • Title
     Comparison of polymerase chain reaction assay and cytotaxonomy for identification of sibling species of Anopheles fluviatilis (Diptera:Culicidae)
    Published in “bulletin of entomological research” (2003) 93, 169-171.
    Abstract: Anopheles fluviatilis is a complex of three species, identified by species specific diagnostic inversions seen in the banding pattern of the polytene chromosomes, hence malaria is endemic in some places and in some places it is absent even though Anopheles fluviatilis is present. The polymerase chain reaction (PCR) assay was developed from rDNA region and two species were identified. Multiplex PCR for these two species was also done.
     Diagnostic kit for the detection of the vector species of
    An. fluviatilis.
    Abstract: Role in malaria transmission varies from place to place. Despite the high density of An. fluviatilis in certain area’s the species has no significant role in malaria transmission while in other area’s the species of An. fluviatilis accounts for 80% of the malaria cases. The polymerase chain reaction (PCR) assay was developed from the rDNA region and two species were identified. Multiplex PCR for these two species was also done. The above probe is proposed to be developed into a kit to minimize the errors arising due to variation in the reagents used in the PCR assay.
     rDNA-PCR assay for the vector species of An.fluviatilis in mosquito pools.
    Abstract: A rDNA based PCR assay was developed to differentiate the vector species of the Anopheles fluviatilis complex which is an important vector of malaria. The assay amplified a diagnostic PCR product of size 350 bp. This probe was able to detect An. fluviatilis in pool size ranging between 50-60 mosquitoes of different genera. Such a study will help in the early detection and effective monitoring of this important malarial vector and its breeding habitat in order to aim at suitable control strategies.
     Development of molecular diagnostic assay for members of the Anopheles culicifacies lato complex for improved malaria vector surveillance.
    Project ongoing: To design and develop diagnostic PCR assay for the identification of individual members of the Anopheles culifacies complex. Also to develop a multiplex PCR assay for all members of the Anopheles culicifacies complex. Different regions of the ribosomal DNA namely ITS1, ITS2, IGS will be amplified by PCR.
     PCR assay for distinguishing An.minimus from its closely related member An. fluviatilis.
    Project ongoing: To design and develop diagnostic PCR assay for the identification of individual members of the Anopheles minimus complex. Also to develop a multiplex PCR assay for all members of the complexes of An. minimus and An. fluviatilis. Different regions of the ribosomal DNA namely ITS1, ITS2, IGS will be amplified by PCR.

    EDUCATION M.Sc Microbiology
    Manasa Gangotri
    University of Mysore
    Mysore, Karnataka
    India

    B.Sc Microbiology
    ST. Agnes College
    University of Mangalore
    Mangalore, Karnataka
    India

    Pre University
    Desheeya Vidya Shala
    Independent (Jr) College,
    Shimoga , Karnataka
    India

    Matriculation
    Mary Immaculate girls high school
    Shimoga, Karnataka
    India

    REFERENCES Dr. Sahar Althawadi, MD, ABMM
    Section Head
    Microbiology Laboratory, MBC 10
    Department of Pathology and Laboratory Medicine,
    King Faisal Specialist Hospital & Research Center
    Tel: (9661) 4424114 Fax: (9661) 4424331
    e-mail: sthawadi@kfshrc.edu.sa

    Hala Imambaccus
    Senior Medical Technologist
    Microbiology Laboratory, MBC 10
    Department of Pathology and Laboratory Medicine,
    King Faisal Specialist Hospital & Research Center
    Tel: (9661) 4647272 ext: 35890
    e-mail: halabaccus@hotmail.com

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